Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of exposures to TBBPA on the phosphorylation/activation state of PKC and PKD in NK cells: Representative experiment for 10 min exposures to TBBPA. Experiment was repeated using cells from seven different donors.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay

Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of incubations with K562 target cells on phosphorylation/activation state of PKC in NK cells: A) levels of phospho-PKC normalized to the control. B) Representative Western blot. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay, Western Blot

Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of incubations with K562 target cells on phosphorylation/activation state of PKD in NK cells: A) levels of phospho-PKD normalized to the control. B) Representative Western blot. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay, Western Blot

Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of exposures to TBT on the phosphorylation/activation state of PKC in NK cells: A) levels of phospho-PKC normalized to the control for 10 min exposures to TBT. B) Representative experiment for 10 min exposures to TBT. C) Representative experiment for 1 h exposures to TBT. D) Representative experiment for 6 h exposures to TBT. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay

Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of exposures to TBT on the phosphorylation/activation state of PKD in NK cells: A) levels of phospho-PKD normalized to the control for 10 min exposures to TBT. B) Representative experiment for 10 min exposures to TBT. C) Representative experiment for 1 h exposures to TBT. D) Representative experiment for 6 h exposures to TBT. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay

Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of exposures to DBT on the phosphorylation/activation state of PKC in NK cells: A) levels of phospho-PKC normalized to the control for 10 min exposures to DBT B) Representative experiment for 10 min exposures to DBT. C) levels of phospho-PKC normalized to the control for the 1 h exposures to DBT. D) Representative experiment for 1 h exposures to DBT. E) Representative experiment for 6 h exposures to DBT. Experiments were repeated using cells from six different donors for 10 min and 1h and form three different donors for 6 h. Asterisks indicate a significant difference as compared with control, p<0.05.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay

Journal: Toxicology mechanisms and methods

Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A

doi: 10.3109/15376516.2015.1070226

Figure Lengend Snippet: Effects of exposures to DBT on the phosphorylation/activation state of PKD in NK cells: A) levels of phospho-PKD normalized to the control for 10 min exposures to DBT. B) Representative experiment for 10 min exposures to DBT. C) Representative experiment for 1 h exposures to DBT. D) Representative experiment for 6 h exposures to DBT. Experiments were repeated using cells from five different donors (10 min), six different donors (1 h) and three different donors (6 h). Asterisks indicate a significant difference as compared with control, p<0.05.

Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of RosetteSep human NK cell enrichment antibody cocktail (Stem Cell Technologies, Vancouver, BC, Canada).

Techniques: Activation Assay

Journal: iScience

Article Title: Epigenetic and transcriptional regulation of CCL17 production by glucocorticoids in arthritis

doi: 10.1016/j.isci.2023.108079

Figure Lengend Snippet:

Article Snippet: Briefly, they were purified from buffy coats, using RosetteSep Ab cocktail (Stem Cell Technologies, Vancouver, BC, Canada), which negatively selects CD14 + monocytes, followed by Ficoll-Paque density gradient centrifugation.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Chromatin Immunoprecipitation, Gene Expression, Software, Protease Inhibitor, Western Blot, Reverse Transcription